منابع مشابه
ExCyto PCR Amplification
BACKGROUND ExCyto PCR cells provide a novel and cost effective means to amplify DNA transformed into competent bacterial cells. ExCyto PCR uses host E. coli with a chromosomally integrated gene encoding a thermostable DNA polymerase to accomplish robust, hot-start PCR amplification of cloned sequences without addition of exogenous enzyme. RESULTS Because the thermostable DNA polymerase is sta...
متن کاملEffect of Lowering PCR Reaction Volumes and Increasing the Speed of DNA Amplification on the Sensitivity of PCR
ABSTRACT Recently, PCR is being reported more frequently with satisfactory results on the diagnosis of clinical infections. Widespread availability of PCR promises a sensitive and specific alternative, to traditional methods, but these benefits must be balanced against cost. Protocols using small volumes have described where the reaction taken place in capillary tubes, and small volumes work...
متن کاملErrors induced during PCR amplification
The Polymerase Chain Reaction (PCR) is one of the most widely used techniques in modern molecular biology to amplify a single or few copies of a piece of DNA sequences. The PCR products are used as inputs of many other applications such as diagnosis of diseases. Therefore the accuracy of the further analysis depends on the quality of the PCR outputs. In order to study the amplification process ...
متن کاملPCR Amplification of Reverse - transcribed mRNA
We reported that interleukin (IL) 6 alone cannot induce osteoclast formation in cocultures of mouse bone marrow and osteoblastic cells, but soluble IL-6 receptor (IL-6R) strikingly triggered osteoclast formation induced by IL-6. In this study, we examined the mechanism of osteoclast formation by IL-6 and related cytokines through the interaction between osteoblastic cells and osteoclast progeni...
متن کاملAn evaluation of direct PCR amplification
AIM To generate complete DNA profiles from blood and saliva samples deposited on FTA® and non-FTA® paper substrates following a direct amplification protocol. METHODS Saliva samples from living donors and blood samples from deceased individuals were deposited on ten different FTA® and non-FTA® substrates. These ten paper substrates containing body fluids were kept at room temperature for vary...
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ژورنال
عنوان ژورنال: Cell Stress & Chaperones
سال: 1997
ISSN: 1355-8145,1466-1268
DOI: 10.1379/1466-1268(1997)002<0276:mapaoh>2.3.co;2